llc mk2 cell sev Search Results


llc mk2 cell sev  (ATCC)
95
ATCC llc mk2 cell sev
Llc Mk2 Cell Sev, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/llc mk2 cell sev/product/ATCC
Average 95 stars, based on 1 article reviews
llc mk2 cell sev - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
noninfectious sev particle expressing envf (nvp-envf)  (ID Pharma Co Ltd)
90
ID Pharma Co Ltd noninfectious sev particle expressing envf (nvp-envf)
Noninfectious Sev Particle Expressing Envf (Nvp Envf), supplied by ID Pharma Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/noninfectious sev particle expressing envf (nvp-envf)/product/ID Pharma Co Ltd
Average 90 stars, based on 1 article reviews
noninfectious sev particle expressing envf (nvp-envf) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
cell line llc mk2  (ATCC)
98
ATCC cell line llc mk2
Cell Line Llc Mk2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell line llc mk2/product/ATCC
Average 98 stars, based on 1 article reviews
cell line llc mk2 - by Bioz Stars, 2026-03
98/100 stars
  Buy from Supplier
cantell  (Fushimi Pharmaceutical)
90
Fushimi Pharmaceutical cantell
Cantell, supplied by Fushimi Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cantell/product/Fushimi Pharmaceutical
Average 90 stars, based on 1 article reviews
cantell - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
qiaamp viral rna mini kit  (Qiagen)
96
Qiagen qiaamp viral rna mini kit
Qiaamp Viral Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/qiaamp viral rna mini kit/product/Qiagen
Average 96 stars, based on 1 article reviews
qiaamp viral rna mini kit - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
opti-mem  (Thermo Fisher)
90
Thermo Fisher opti-mem
Opti Mem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opti-mem/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
opti-mem - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
sendai virus strain 52 (sev-52  (Charles River Laboratories)
90
Charles River Laboratories sendai virus strain 52 (sev-52
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Sendai Virus Strain 52 (Sev 52, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sendai virus strain 52 (sev-52/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
sendai virus strain 52 (sev-52 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
hela  (ATCC)
99
ATCC hela
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Hela, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hela/product/ATCC
Average 99 stars, based on 1 article reviews
hela - by Bioz Stars, 2026-03
99/100 stars
  Buy from Supplier
superfect reagent  (Qiagen)
90
Qiagen superfect reagent
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Superfect Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superfect reagent/product/Qiagen
Average 90 stars, based on 1 article reviews
superfect reagent - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lipofectamine  (Thermo Fisher)
86
Thermo Fisher lipofectamine
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Lipofectamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipofectamine/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
lipofectamine - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier
alexa fluor 594  (Thermo Fisher)
86
Thermo Fisher alexa fluor 594
A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of <t>SeV</t> <t>52</t> per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.
Alexa Fluor 594, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 594/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
alexa fluor 594 - by Bioz Stars, 2026-03
86/100 stars
  Buy from Supplier

Image Search Results


A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of SeV 52 per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.

Journal: bioRxiv

Article Title: Murine Parainfluenza Virus Persists in Lung Innate Immune Cells Sustaining Chronic Lung Pathology

doi: 10.1101/2023.11.07.566103

Figure Lengend Snippet: A. Timeline of the study design. Mice were inoculated with either phosphate-buffered saline (mock) or 5×10 4 tissue culture infectious dose (TCID 50 ) of SeV 52 per animal. Lungs were analyzed on days 3 and 49 post-infection. B. Disease progression was monitored by measuring weight loss through the experiment. Data are representative of 4 independent experiments (mean ±SD). For SeV-infected and mock groups the area under the curve (AUC) was calculated and t-tests were performed for statistical significance analysis. ****P<0.0001. C. Whole lung homogenates were harvested at days 3 and 49 post-infection and both SeV NP RNA expression and infectious virus titers were quantified by qPCR and infectivity assays respectively. Relative RNA quantitation by qPCR was normalized to mouse GAPDH and β-Actin. Two-way analysis of variance with Holm-Sídák post-test was used to estimate statistical significance between groups. N = 5 animals per group. *P<0.05; **P<0.01; ****P<0.0001. D. SeV-infected lungs were stained for SeV NP (white staining, upper panels) using immunofluorescence and for SeV NP RNA using RNAscope (white staining, lower panels). Nuclear staining (Hoechst for immunofluorescence and DAPI for RNAscope) in blue. Representative images of 3 independent experiments. Scale bars: 100 µm. E. SeV-infected lungs were stained for basal stem cells (Krt5 + , green staining) and SeV NP (magenta staining) to localize SeV NP + cells in relation to areas displaying chronic lesions (dashed areas, subpanels E1-E3) and unaffected areas (subpanel E4). Arrowheads indicate SeV NP + cells, more detailed in the correspondent zoomed inset panels. Images were taken using a widefield microscope. Left panel, tiling image, 5x magnification. Right subpanels, 20x magnification. Right insets: digital zooms from the correspondent 20x magnification images. Scale bars: Left panel. 500 µm; Subpanels. 100 µm. Images are representative of 3 independent experiments, 5 mice per group.

Article Snippet: Sendai virus strain 52 (SeV-52) stocks were expanded in 10-days-old embryonated chicken eggs (Charles River Laboratories, Wilmington, MA) and virus titers were determined using end-point dilution tissue culture infectious dose (TCID 50 ) infectivity assays in LLC-MK2 cells.

Techniques: Saline, Infection, RNA Expression, Virus, Quantitation Assay, Staining, Immunofluorescence, Microscopy